Protein L: An Immunoglobulin Light Chain-binding Bacterial Protein
نویسندگان
چکیده
منابع مشابه
Protein L: an immunoglobulin light chain-binding bacterial protein. Characterization of binding and physicochemical properties.
Protein L, a cell wall molecule of the bacterial species Peptostreptococcus magnus with affinity for immunoglobulin (Ig) light chains, was isolated after solubilization of the bacterial cell walls with mutanolysin or from the culture medium by a single affinity chromatography step on human IgG-Sepharose. A major protein band with an apparent molecular weight of 95,000 was obtained from both sou...
متن کاملStructure of peptostreptococcal protein L and identification of a repeated immunoglobulin light chain-binding domain.
The gene for protein L, an immunoglobulin (Ig) light chain-binding protein expressed by some strains of the anaerobic bacterial species Peptostreptococcus magnus, was cloned and sequenced. The gene translates into a protein of 719 amino acid residues. Following a signal sequence of 18 amino acids and a NH2-terminal region ("A") of 79 residues, the molecule contains five homologous "B" repeats o...
متن کاملImmunoglobulin heavy chain and binding protein complexes are dissociated in vivo by light chain addition
Immunoglobulin heavy chain binding protein (BiP, GRP78) associates stably with the free, nonsecreted Ig heavy chains synthesized by Abelson virus transformed pre-B cell lines. In cells synthesizing both Ig heavy and light chains, the Ig subunits assemble rapidly and are secreted. Only incompletely assembled Ig molecules can be found bound to BiP in these cells. In addition to Ig heavy chains, a...
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Identification of the immunogens responsible for the production of pathogenic autoantibodies is a major problem in the field of autoimmunity. In the principal diseases that are thought to involve autoimmunization, rheumatoid arthritis, type I diabetes, and multiple sclerosis are examples, the instigating stimulus is unknown. Another example of the gap in our knowledge of disease-causing immunog...
متن کاملEvidence for plasticity and structural mimicry at the immunoglobulin light chain-protein L interface.
The multidomain bacterial surface protein L (PpL) is a virulence factor expressed by only 10% of Peptostreptococcus magnus strains, and its expression is correlated with bacterial vaginosis. The molecular basis for its ability to recognize 60% of mammalian immunoglobulin light chain variable regions (V(L)) has been described recently by x-ray crystallography, which suggested the presence of two...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1989
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(19)47174-3